一、Human FXR ELISA 试剂盒的产品简述：
ELISA 品 牌：USCN
ELISA 货 号：072042h
ELISA英文名：Human FXR ELISA Kit
ELISA中文名：人的法尼酯X受体，FXR ELISAkit
二、 人法尼酯X受体ELISA kit的技术参数：
1. 检测范围：
2. 灵 敏 度：
3. 适用标本：
4. 保存条件：2-8℃
5. 有 效 期：6个月
三、人FXR ELISA试剂盒的产品用途：
产品用途：酶联免疫实验(ELISA实验)
备 注：仅供参考，请以随货纸质说明书为准，更多产品如抗体(antibody)；蛋白(protein)；鲎试剂；内毒素工作标准品；内毒素指示剂；无热原检查用水；无热原吸咀；紫外灯；色谱仪；色谱柱等更详细信息请登录上海武昊公司网站禁发网址wuhaosh禁发网址 或致电咨询。
ELISA实验前，血清、血浆、组织、细胞培养上清等样品的采集及处理
Serum- Use a serum separator tube and allow samples to clot for two hours at room temperature or overnight at 4oC before centrifugation for 20 minutes at approximately 1000×g. Assay freshly prepared serum immediately or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.
Plasma- Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 000×g at 2 - 8oC within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.
Tissue homogenates - The preparation of tissue homogenates will vary depending upon tissue type. For this assay, tissues were rinsed in ice-cold PBS(0.02mol/L,pH 7.0-7.2) to remove excess blood thoroughly and weighed before homogenization. Minced the tissues to small pieces and homogenized them in 5-10 mL of PBS with a glass homogenizer on ice(Micro Tissue Grinders woks, too). The resulting suspension was sonicated with an ultrasonic cell disrupter or subjected to two freeze-thaw cycles to further break the cell membranes. After that, the homogenates were centrifugated for 5 minutes at 5000×g. Remove the supernate and assay immediately or aliquot and store at ≤-20oC.
Cell culture supernates - Centrifuge samples for 20 minutes at 1000×g. Remove particulates and assay immediately or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.
